SPF AnalyzerTM

Download Brochure

spf testing

SPF AnalyzerTM

 

 

 

 

 

 

 

 

 

 

 

 

 

Introduction

Introduction

SPF-290AS Turnkey Automated Analyzer

SPF-290AS Sunscreen Protection Factor Analyzer is delivered as an integrated turnkey system packaged in a self-contained, attractive desktop unit. The SPF-290AS Computer includes a CPU, keyboard, monitor, mouse, Windows® operating system, data acquisition board and drivers, I/O, communication and power cables and WinSPF operating software and complete instruction manual. WinSPF v4.1 operating software is installed and tested and includes complete on-line help system, audio-visual training aid, COLIPA spreadsheet, fully functional toolbar, single step control icons, graphical and tabular data display formats and utilizes all Windows® multi-tasking features. Available for use with 110V AC, 60 Hz, or 220V AC, 50 Hz.

Each SPF-290AS Sunscreen Protection Factor Analyzer is also supplied with a Validation Kit enabling the operator to perform comprehensive testing of the analyzer to ensure accurate spectral and signal dynamic range performance.

To begin immediate testing at system delivery, a 50 x 50 mm PMMA plate holder, along with a sample set of three 5μ roughness PMMA plates and a box of 25 syringes for sample application are also provided. Depending on your intended testing methods, additional boxes of two hundred PMMA plates in either 2μ roughness or 5μ roughness are available. As an option, 3M™ Transpore™ tape and Transpore tape holders are also available for performing less demanding measurement data collection.

100′s of Customers Worldwide including…

Avon, BASF, Clairol, Elizabeth Arden, Estee Lauder, Good Housekeeping, ISP, Johnson & Johnson, Lever Bros, L’Oreal, Mary Kay, Merck, Revlon, Rohm & Haas, Proctor & Gamble, Unilever, Andrew Jergens, Helene Curtis, Whitehall Robins, Bayer, S.C. Johnson, Bristol-Myers Squibb, Tanning Research, Pfizer, Boots the Chemist UK Ltd. and Amway…to name just a few!

SETTING THE STANDARD FOR THE EFFICIENT,

LOW COST, IN-VITRO SPF ANALYSIS OF -

  • • Liquids
  • • Textiles
  • • Lotions
  • • Creams
  • • Sprays
  • • Gels
  • • Powders
  • • Emulsions

Automated, and Easy to Operate

Automated, and Easy to Operate

Fully Automated Multi-Point Sampling - Saves Time and Money!

While other analyzers are limited to single position measurements, requiring the operator to manually reposition the sample for each measurement position, the SPF-290AS enables simple push button Hit and Forget Automated Sequencing of up to 12 sampling positions such as required in the COLIPA methods. With Hit-and-Forget Automated Sequencing, this time saving feature lets the operator walk away from the analyzer and work on other tasks while the SPF-290AS completes the methods’ required multiple position measurement routine automatically.

Two modes of automated operation:

Data can be acquired in approximately 20 seconds. Results displayed in easy to read format.

Autoscan

Autoscan mode offers two methods of choosing up to 12 sampling locations. The operator can either specify the positions to be read or the computer can generate them randomly. Once set, the operation, data collection and reporting are performed automatically. The stage moves the sample on the holder into the light beam, takes the measurements, moves to the next position and continues until all the measurements have been completed.

Automated “Hit and Forget” operation:

• Improves repeatability and accuracy of measurements

• Eliminates the need to manually reposition the sample for each measurement and allows operators to perform other functions

• Enables time based photo-stability measurements

• Get to market sooner

• Reduces the cost of testing

• New sample pattern for PMMA plates

 

Photo-Stability

Time-based measurements monitor the SPF values for a sample at a user-specified position against time. The effects of drying and exposure to air and light on a sample can be easily evaluated making it particularly suitable for photostability testing. The system’s computer provides a controlled repeatable time base for measurements.

Intuitive Software Features

Intuitive Software Features

Easy to use, and intuitive to operate, WinSPF operating software allows simple access to perform a multitude of standard and custom measurement methods and reporting functions. The analyzer is delivered complete with Audio Visual Training aids, COLIPA spreadsheet, complete on-line help system, fully functional toolbar, single-step control icons, graphical and tabular data display formats and utilizes all Windows® multi-tasking features.

Thorough report routines generate essential data tracking information at the press of a button.

Document:

• Date

• Time

• Operator

• Sample ID

• Test Parameters

• And more

Many additional software features include:

• Utilizes All Windows Features: Printing, Screen Capture, Etc.

• Complete On-Line Help System

• Fully Functional Toolbar

• System Test and Validation

• Switch between display formats

• Single step control icons

• Critical wavelength calculation

• Calculate and print SPF values

• Both tabular and graphical formats

• Boots Star Rating

• Create formulation assays of up to 36 scans

• Display MPF and absorbance values

• Photo-stability testing

• Calculate area under the curve

• UVA/UVB ratio

• View scans individually

• Calculates TNUV standard

• Supports FDA UVA in-vitro test procedure

• Computation of statistics for multiple assays

Validation Kit

Validation Kit

An SPF-290AS Validation Kit is included with each analyzer and comes complete with a Comprehensive Test Plate. The Comprehensive Test Plate contains several optical filters that are measured by the SPF-290AS and the results are compared to NIST traceable factory measurements. This feature is designed to provide a clear understanding and validation of how your SPF-290AS analyzer is performing against factory measurements for optical density, wavelength accuracy, and correlation to in-vivo testing whenever desired.

Equipped with this tool, users will not spend time performing tests on an analyzer that is in need of repair, nor will they spend money for repairs that are not necessary.

The SPF-290AS Validation Kit includes:

• Storage case

• 10 syringes with tips, 1 ml, disposable

• PMMA plates, 50 x 50 mm, 5μ roughness, sample set of 3

• Sample holder for 50 x 50 mm PMMA plates

• Calibration Filter Plate assembly

• 1 oz jar of Homosalate Standard

• 1 oz jar of COLIPA Standard

• Factory calibration data

High Correlation with In-vivo Testing

High Correlation with In-vivo Testing

The high correlation between the SPF-290AS in-vitro measurements and in-vivo test results provides confidence that the analyzer will be a reliable guide to product performance. The SPF-290AS delivers reliable results on the most difficult samples. An easy-to-use testing methodology combined with reproducible results opens the door to efficient and low cost experimental design techniques for formulation optimization. This will result in faster formulation and lower development costs due to a reduction in the need for extensive in-vivo panel studies.

Korea Food & Drug Administration Qualify the SPF-290S as an Alternative Method to in-vivo Testing

The results of this study for correlation between the in-vivo SPF and in-vitro SPF measured using SPF-290 analyzer are…..The in vitro SPF test method will be used as an alternative method for in vivo SPF and a new test item for quality control sunscreens.

- Drug Evaluation Department, Korea Food & Drug Administration, 5 Nokbun-dong, Eunpyung-gu, Seoul, Korea

Sampling Handling & Support Media

Sampling Handling & Support Media

Studies have shown the critical importance of sample preparation, including accuracy of sample volume, and uniformity of sample spreading over the plate surface, as well as the affect that the different types of sample plates have on test results. Measurement results should not be compared if prepared and analyzed on non-similar PMMA plates and 3M™ Transpore™ tape.

Solar Light recommends the use of Schonberg PMMA plates for high accuracy and correlation to in-vivo testing.

NOTE: Please contact Solar Light and we will be happy to provide links to professional studies reporting on spreading methods and the revised COLIPA method discussing the importance of the newer 5 micron roughness plates being offered.

Industry recommends sample materials be deposited on a PMMA substrate in a high number of small droplets, and then evenly spread by a finger tip over the substrate. The uniformity and consistency of this process directly correlates to your results. Refer to the following table for examples.

Test Parameter COLIPA 2007 COLIPA 2011 ISO 24443
Plate Roughness Low Roughness High Roughness High Roughness
Application Amount 0.75 mg/cm2 1.3 mg/cm2 1.3 mg/cm2
Application Dosing Small droplets Small droplets Small droplets
Spreading Method Finger tip Finger tip Finger tip
Spreading Sequence 1 Quickly, without pressure Quickly, without pressure Quickly, small circular motions, with minimal pressure
Spreading Sequence 2 Rubbing into the surface, using pressure Rubbing into the surface, using pressure Alternating horizontal and vertical strokes, with moderate pressure

 

A common and often successful technique for applying sample material to the PMMA plate is via a simple disposable syringe.

Since measurement methods are now directing the dispensed dose by weight, these low cost applicators can be fine, though somewhat challenging in the application of equal volume of a large number of small droplets. An easy, accurate, and consistent method for uniform droplet dosing is through a precision positive displacement pipette.

Solar Light offers an optional sophisticated solution for repetitive liquid handling applications. The manual pipette hand dispensers are ideal for process acceleration and saving time on long-series pipetting. Their increased volume selection provides outstanding flexibility and exceptional support for research as well as routine tasks, and their smooth handling reduces the fatigue associated with repetitive work. Inexpensive disposable tips (replacement capillaries and pistons) easily attach to the pipette, and a built-in sensor recognizes the size of the tip, and the volume appears automatically in the display—eliminating time-consuming volume calculations and incorrect dispensing volumes.

The tips function according to the positive-displacement principle, which ensures that the correct volume is dispensed regardless of the density or viscosity of the liquid. The tips are easily replaced, eject with the push of a button, and dispose after use.

Features

• Easy to read: automatic disposable tip recognition with electronic volume display

• Easy volume setting

• 20 different dispensing volumes from 1 µL-50 ml

• Two-year warranty

 

PMMA plates packaged in boxes containing 200 each are available in Low Roughness 2µm, and High Roughness 5 µm surface formats.

What is Delivered

What is Delivered

SPF-290AS Turnkey Automated Analyzer

SPF-290AS Sunscreen Protection Factor Analyzer is delivered as an integrated turnkey system packaged in a self-contained, attractive desktop unit. The SPF-290AS Computer includes a CPU, keyboard, monitor, mouse, Windows® operating system, data acquisition board and drivers, I/O, communication and power cables and WinSPF operating software and complete instruction manual. WinSPF v4.1 operating software is installed and tested and includes complete on-line help system, audio-visual training aid, COLIPA spreadsheet, fully functional toolbar, single step control icons, graphical and tabular data display formats and utilizes all Windows® multi-tasking features.  Available for use with 110V AC, 60 Hz, or 220V AC, 50 Hz.

Each SPF-290AS Sunscreen Protection Factor Analyzer is also supplied with a Validation Kit enabling the operator to perform comprehensive testing of the analyzer to ensure accurate spectral and signal dynamic range performance.

To begin immediate testing at system delivery, a 50 x 50 mm PMMA plate holder, along with a sample set of three 5μ roughness PMMA plates and a box of 25 syringes for sample application are also provided. Depending on your intended methods, additional boxes of two hundred PMMA plates in either 2μ roughness or 5μ roughness are available. As an option, 3M™ Transpore™ tape and Transpore tape holders are also available for performing less demanding measurement data collection.

The Complete SPF-290AS Sunscreen Protection Factor Analyzer and Computer:

• Motorized and automated X-Y sample stage and control module for Hit-and-Forget measurements of up to 12 discrete sample positions

• 125W CW Xenon source and precision integrating sphere

• Color compensating filter

• PMT detection module – The photomultiplier provides extremely high sensitivity and ultra-fast response. Photomultiplier tubes (PMT) have high bandwidth and noise-free gain on the order of a million. This makes PMTs ideal for the detection of extremely low light levels as required in SPF measurements.

• Precision scanning monochromator with stepping motor and controller

• Computer, including;a. CPUb. Keyboardc. Monitord. Mousee. Windows® operating systemf. Data acquisition boardg. Driversh. WinSPF v4.1 software installed and tested. Win­SPF operating software also includes complete on-line help system, fully functional toolbar, single step control icons, graphical and tabular data display formats and utilizes all Windows® multi-tasking features.i. Communication and power cables

• Box of 25 syringes with tips, 1 ml, disposable

• Validation kit, including;a. Storage caseb. 10 syringes with tips, 1 ml, disposablec. PMMA plates, 50 x 50 mm, 5μ roughness, sample set of 3d. Sample holder for 50 x 50 mm PMMA platese. Calibration Filter Plate assemblyf. 1 oz jar of

• Homosalate Standardg. 1 oz jar of COLIPA Standardh. Factory calibration data

• Complete instruction manual

• Audio/ Visual training

• Available for use with 110V AC, 60 Hz, or 220V AC, 50 Hz. Specify at time of order.

 

Specification and Performance Guideline

Specification and Performance Guideline

Wavelength Range 290 – 400 nm (range specified by international methods)
Wavelength Accuracy 0.2% (0.58 nm – 0.80 nm)
Wavelength Reproducibility 0.25 nm
Spectral FWHM Bandwidth 1.66 nm
Absorbance 0 – 3.2 A (Dual Doped PMMA Method)
SPF Measurement Range 1 – 50+
Scan Time As little as 24 seconds
Wavelength Step Interval 1 nm, 2 nm, or 5 nm. User selectable.
Lamp Power stabilized Xenon CW125 W (operated at 75 W)
Detector High Sensitivity Multialkali side-on PMT
Sample Positioning Automated X-Y Stage
Power Options 110V AC, 60 Hz220V AC, 50 Hz
Current Methods Include COLIPA/ISO in-vitro method for UVA-PF/SPF ratio (for EU labeling)
Critical Wavelength (for EU Labeling)Boots Star Rating (for EU Labeling)
FDA Critical Wavelength (for US Labeling)Monograph FDA 21 CFRAATCC-183 Fabric Test Method
AS NZS 4399 Sun Protective Clothing Standard, SPF ValuesAustralian TNUV Standards
UVA/UVBUVA Protective Factor CalculationsErythemal Protection Factor calculations,
ISO: 24443 Untreated substrate measurement and data storage
Warranty 1 Year

Measurement

Measurement

The SPF-290S is a recording UV spectrophotometer designed and optimized for the determination of SPF values on a variety of sunscreen and cosmetic products reducing the need (and cost!) for in-vivo testing.

Covering both the UVB and UVA spectral regions, the system automatically scans from 290 to 400 nm, accumulating and storing data at intervals of 1, 2 or 5nm. The monochromatic protection factor (MPF) is determined for each of the selected wavelengths and is used to calculate the SPF value, using solar irradiance and erythemal constants that are programmed into the software but which can be easily modified.